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KMID : 1007519990080010024
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Food Science and Biotechnology
1999 Volume.8 No. 1 p.24 ~ p.29
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Isolation of Intracellular Inulinase - Producing Bacterium and Culture Condition for Inulinase Production
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Kwon, Su Jin
YOO, JIN YOUNG/Lee, Jong Soo
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Abstract
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A bacterium (188-1) which produces a high level of intracellular inulinase was isolated from soil and identified as a strain of Bacillus sphaericus upon investigation of the morphological and physiological characteristics. Synthesis of inulinase by B. sphaericus 188-1 was mainly induced by inulin and an optimal medium for the enzyme production was established as 0.5% inulin, 0.4% peptone, and 10 mM MgSO₄·7H₂O (pH 7.5). The production of inulinase reached the highest level of 286 unit per ㎎ protein after 10 hr cultivation by 7 L fermentor at 30℃.
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